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Virus found in germ cells of COVID-19 patients - sexual transmission possible; in line with studies on testis damage and lower male gonadal function – Healthnews21 (part of Southern Media Corp.), from an exclusive by Sci. and Tech. Express News (part of Shanghai United Media Group, largest in China)

Update: Aveolar Macrophage Activation and Cytokine Storm in the Pathogenesis of Severe COVID-19 - Ruijin Hospital, Shanghai Jiaotong University School of Medicine (Mar 25, 2020)
An important finding in the present work was the infections of...spermatogenic testicular cells (Extended Data Fig.4k, 100×) by SARS-CoV–2 without obvious histological abnormalities. In addition, ...spermatogenic Sertoli and Leydig cells were all infected by SARS-CoV–2 (Extended Data Fig.4j, l, 400×).
Virus Found in Germ Cells of COVID-19 Patients: Possible Sexual Transmission; in Line with Studies on Testis Damage and Lower Male Gonadal Function
Original title: Bombshell: Virus found in germ cells of COVID-19 patients! May be sexually transmittable?
Mar 30, 2020 22:06 | Healthnews21 (part of Southern Media Corporation)|by Writer Tang Wuike and Editor Li Xinyi
According to the exclusive coverage by Science and Technology Innovation Express News (part of China’s largest media group, Shanghai United Media Group) on Mar 28, Ning Guang, Director of Ruijin Hospital in Shanghai shared at “Fundamental Studies and Infection Prevention and Control of Virus Mutation, Evolution, Transmission Conference” germ cells of COVID-19 patients are found to be infected by the SARS-CoV-2 virus, so sexual contact may be one of methods of transmission.
On one hand, maternal-fetal and fecal-oral transmissions etc. have not been ruled out; on the other hand, sexual transmission comes to sight. As the COVID-19 pandemic develops, more and more academicians initiated their in-depth studies. Not long ago was there an alarming and bewildering study suggesting that the SARS-CoV-2 virus possibly damages kidneys and testes. Would contracting COVID-19 really sterilize men then?
Sexual contact also a method of transmission of COVID-19?
Germ cells include spermatogenous cell, primary spermatocyte, secondary spermatocyte, spermatogonia, and sperms. Testes are where the process of spermatogenesis - aploid spermatozoa develop from germ cells takes place.
In the aforementioned conference, Ruijin Hospital Director Ning Guang pointed out infection by SARS-CoV-2 in the germ cells of COVID-19 patients, showing that the disease could possibly be sexually transmissible. Sexual transmissions typically mean direct and indirect transmissions of sexual diseases – transmission through sexual contact or from parents to infant.
In the same vein, there was a study on sexual transmission potential of SARS-CoV-2 infected female patients by the Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology in Wuhan.
From January 28 to February 18, 2020, 35 female patients diagnosed with COVID-19 in Tongji Hospital were included in this descriptive study. The gynecologic history, clinical characteristics, laboratory findings and chest computed tomography (CT) of all patients were recorded in detail.
Real-time polymerase chain reaction testing (RT-PCR) was employed to detect SARS-CoV-2 in vaginal environment (including vaginal discharge, cervical or vaginal residual exfoliated cells) and anal swab samples, and inquired recent sexual behaviors from the patients. All the samples from vaginal environment were negative for SARS-CoV-2; only an anal swab sample from one patient was positive for the virus.
No evidence of sexual transmission of SARS-CoV-2 was discovered in this study. Nevertheless, one of the authors, Prof. Wang Shixuan stated during an interview with Red Star News (under Chengdu Economic Daily) that the result might be related to the lack of ACE2 expression, the receptor of SARS-CoV-2, in cervical and vaginal residual exfoliated cells.
Multiple potential ways of transmission still worth attention
Apart from sexual transmissions, previous studies and observations from clinical cases also hinted at the potential of maternal-fetal and fecal-oral transmissions. It is already discovered SARS-CoV-2 can stay alive for rather long in feces – a patient whose stool samples tested positive for 47 days straight30083-2/fulltext), but it is still not clear whether the virus can be spread via fecal-oral transmission.
At present, many provinces and cities have adopted stricter standards for discharging patients. In addition to fulfilling criteria for ending quarantine set in COVID-19 Diagnostics and Treatment Scheme (7th Edition), patients are discharged only after their anal swab sample tested negative.
Recently, Journal of the American Medical Association also published several studies on possible vertical transmission, and added a banner on its website homepage to remind its risks.
Serology diagnostic criteria in COVID-19 Diagnostics and Treatment Scheme (7th Edition) include positive result for SARS-CoV-2 N-Protein IgM antibodies 3 to 5 days after onset, and IgG antibody level in the recovering phase rises four times or higher than in the acute phase. Due to the larger size of IgM antibodies, they are not likely to enter the fetus through placenta; but if SARS-CoV-2 does, then the fetus may produce IgM antibodies.
Teams from Renmin Hospital of Wuhan University and Zhongnan Hospital of Wuhan University reported newborn babies from SARS-CoV-2 infected mothers tested positive for SARS-CoV-2 antibodies. Although unable to rule out infection during labor, but the newborns’ IgM antibody levels rose within 2 hours of birth, suggesting infection inside the womb. However, sample sizes of these two studies are limited, and they do not involve tests of the amniotic fluid, placentas, cord blood, etc. So maternal-fetal transmission still requires further study.
Bottom line remains COVID-19 is certainly transmitted via droplets and close contact, which are more frequent in daily life, so we must stay vigilant of these main methods of transmission. Speaking of probability, even it is indeed sexually transmissible, but one is most likely infected by the more common ways before sexual intercourse or other intimate acts.
SARS-CoV-2 may impact male fertility
Assumption of possible sexual transmission from this new discovery of infection in germ cells matches incidentally with the reached conclusion of probable testicular damages. As mentioned, testes are sperm-producing organs vital to maintain the health of the male reproductive system. SARS-CoV-2 S uses ACE2 to enter cells, and unfortunately, a NIH study highlighted the high ACE2 expression in spermatogenous cells, Sertoli cells, and Leydig cells. Simply put, the virus is highly likely thwarts male fertility.
Currently, there are multiple studies on effects of SARS-CoV-2 infection upon male gonadal function on pre-print platform medRxiv. One of them by Wuhan University compared the sex-related hormones between 81 reproductive-aged men with SARS-CoV-2 infection and 100 age-matched healthy men, and found that serum luteinizing hormone (LH) was significantly increased, but the ratio of testosterone (T) to LH and the ratio of follicle stimulating hormone (FSH) to LH were dramatically decreased in males with COVID-19.
Another study by the Affiliated Suzhou Hospital of Nanjing Medical University further suggested possible kidney damage from COVID-19 infection. It summarized the clinical data of three previous studies to extract the incidence of abnormal renal function or kidney damage in 6, 41, and 99 patients infected with SARS-CoV-2. Outcome in two relatively larger sample studies suggest that about 3% to 10% of patients had abnormal renal function, including elevated creatinine or urea nitrogen. In addition, 7% of patients experienced acute renal impairment.
Hubei provincial government also brought public attention to the reminder for male patients to test their fertility upon recovery from Prof. Yu-Feng Li in the Department of Obstetrics and Gynecology at Tongji Hospital Affiliated to Tongji Medical College of HUST. But the notice was taken down afterward and the team stated relevant studies are underway.
Be it as it may, male comrades need not panic. Most studies are published on pre-print platforms and yet have been peer-reviewed, so the analyses and conclusions are still up for debate. Lastly, even there are high levels of ACE2 in testicles and kidneys, but it does not necessarily mean the gonads are the first under attack or the hardest hit. Potential adverse effects of male fertility from COVID-19 await deeper investigations and verification.
submitted by k_e_luk to China_Flu

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